Interrogating the impact of aggregation‐induced emission nanoparticles on in vitro protein stability, ex vivo protein homeostasis, and in vivo biocompatibility

Aggregation-induced emission (AIE) materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical photochemical properties. A key step toward translational applications for AIE is to systematically vigorously evaluate biosafety biocompatibility. While previous studies focus on cellular viability toxicity, the impact of detailed stress responses manifesting fitness has been less explored. Herein, this work provides first piece evidence support amphiphilic functionalization nanoparticles minimizes deterioration proteome stability protein homeostasis (proteostasis). To end, four scaffolds molecules were prepared, further functionalized into eight with two shells respectively, characterized physicochemical Thermal shift assay quantitatively demonstrates that after enhance thermodynamic ameliorate aggregation propensity lysate, echoed by cell fractionation experiments. Intriguingly, poor polydispersity index (PDI) exaggerates retention cell. Comparative proteomic analysis uncovers can minimize network. Finally, vigorous interrogation mice model reveals complexity factors affecting biocompatibility profiles vivo, including materials’ size, PDI, treatment frequencies. Overall, necessary maintain balance homeostasis.